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Methods in Physiology Series
This
series describes experimental techniques in cellular, molecular and
general physiology. Each book is edited by experts in their field and
covers theory and history behind the method, critical commentary, major
applications with examples, limitations and extensions of each technique,
and vital future directions.
Volume III
Physiology of Inflammation
Edited by Klaus Ley
This advanced text covers the physiological processes relevant to
inflammation. Although the problem is addressed from a vascular perspective,
pathological aspects are also considered. The book centers on the
recruitment of leukocytes to sites of injury and infection, their function
in the tissue, and the eventual resolution of inflammation. It will be of
use to a wide range of readers: graduate students, postdoctoral fellows, and
physicians and scientists in physiology, biomedical engineering, pathology,
immunology, infectious disease, and related disciplines.
2001, 561 pp.; ISBN 019-512829-X
$95.00 $50.00, APS Member price:
$61.75 $30.00
Ordering information is available from APS Customer Service.
Volume IV
Methods in Cellular Imaging
Edited by Ammasi Periasamy
This well-illustrated book targets graduate students and scientists who
are new to the state-of-the-art fluorescence microscopy techniques used in
biological and clinical imaging. It explains basic concepts and imaging
procedures for wide-field, confocal, multiphoton excitation, fluorescence
resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging,
fluorescence recovery after photobleaching (FRAP), optical tweezers, total
internal reflection, high spatial resolution atomic force microscopy (AFM),
and bioluminescence imaging for gene expression. The usage of these
techniques in various biological applications, including calcium, pH,
membrane potential, mitochondrial signaling, protein-protein interactions
under various physiological conditions, and deep tissue imaging, is clearly
presented.
2001, 432 pp.; 101 illus., ISBN 019-513936-4
$99.50 $50.00, APS Member price
$64.75 $30.00
Ordering information is available from APS Customer Service.
Molecular Imaging
FRET Microscopy and Spectroscopy
Edited by Ammasi Periasamy and Richard N. Day
The detection and measurement of the dynamic
interactions of proteins within the living cell are critical to our
understanding of cell physiology and pathophysiology. With FRET microscopy
and spectroscopy techniques, basic and clinical scientists can make such
measurements at very high spatial and temporal resolution. But sources of
background information about these tools are very limited, so this book
fills an important gap. It covers both the basic concepts and theory behind
the various FRET microscopy and spectroscopy techniques, and the practical
aspects of using the techniques and analyzing the results. The critical
tricks for obtaining a good FRET image and precisely quantitating the
signals from living specimens at the nanomolecular level are explained.
Valuable information about the preparation of biological samples used for
FRET image analysis is also provided. The methods covered include different
types of microscopy systems and detectors (wide-field, confocal,
multi-photon) as well as specialized techniques such as photobleaching FRET,
FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time
and image correlation spectroscopy. Starting from the basics, the chapters
guide readers through the choice of probes to be used for FRET experiments
and the selection of the most suitable experimental approaches to address
specific biological questions. Up-to-date, consistently organized, and
well-illustrated, this unique book will be welcomed by all researchers who
wish to use FRET microscopy and spectroscopy techniques.
August 2005, 304 pp.; 16 b&w and 44 color photos, 49
line illus.,
ISBN 019-517720-7; $108.00, APS Member price: $70.20
To
order this book, contact Elsevier Customer Service (phone:
1-800-545-2522). To receive your 35% membership discount, please
reference discount code
93575 when ordering.
To Order a Book
Information for ordering a specific book(s) is available from
APS Customer Service.
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